Nitrogen-fertilizer addition to an agricultural soil enhances biogenic non-extractable residue formation from 2-13C,15N-glyphosate.

Glyphosate and nitrogen (N) or (P) phosphorus fertilizers are often applied in combination to agricultural fields. The additional P or N supply to microorganisms might drive glyphosate degradation towards sarcosine/glycine or aminomethylphosphonic acid (AMPA), and consequently determine the speciation of non-extractable residues (NERs): harmless biogenic NERs (bioNERs) or potentially hazardous xenobiotic NERs (xenoNERs). We therefore investigated the effect of P or N-fertilizers on microbial degradation of glyphosate and bioNER formation in an agricultural soil. Four different treatments were incubated at 20 °C for 75 days as follows; I: no fertilizer (2-13C,15N-glyphosate only, control), II: P-fertilizer (superphosphate + 2-13C,15N-glyphosate, effect of P-supply), III: N-fertilizer (ammonium nitrate + 2-13C,15N-glyphosate, effect of N-supply) and IV: 15N-fertilizer (15N-ammonium nitrate + 2-13C-glyphosate, differentiation between microbial assimilations of 15N: 15N-fertilizer versus 15N-glyphosate). We quantified 13C or 15N in mineralization, extractable residues, NERs and in amino acids (AAs). At the end, mineralization (36-41 % of the 13C), extractable 2-13C,15N-glyphosate/2-13C-glyphosate (0.42-0.49 %) & 15N-AMPA (1.2 %), and 13C/15N-NERs (40-43 % of the 13C, 40-50 % of the 15N) were comparable among treatments. Contrastingly, the 15N-NERs from 15N-fertlizer amounted to only 6.6 % of the 15N. Notably, N-fertilizer promoted an incorporation of 13C/15N from 2-13C,15N-glyphosate into AAs and thus the formation of 13C/15N-bioNERs. The 13C/15N-AAs were as follows: 16-21 % (N-fertilizer) > 11-13 % (control) > 7.2-7.3 % (P-fertilizer) of the initially added isotope. 2-13C,15N-glyphosate was degraded via the sarcosine/glycine and AMPA simultaneously in all treatments, regardless of the treatment type. The percentage share of bioNERs within the NERs in the N-fertilized soil was highest (13C: 80-82 %, 15N: 100 %) compared to 53 % (13C & 15N, control) and to only 30 % (13C & 15N, P-fertilizer). We thus concluded simultaneous N & glyphosate addition to soils could be beneficial for the environment due to the enhanced bioNER formation, while P & glyphosate application disadvantageous since it promoted xenoNER formation.

Fate of glyphosate and its degradation products AMPA, glycine and sarcosine in an agricultural soil: Implications for environmental risk assessment.

Glyphosate can be biodegraded via the aminomethylphosponic acid (AMPA) and the sarcosine/glycine pathway leading to the formation of three intermediate products AMPA, sarcosine or glycine. The fate of the three intermediate compounds of glyphosate biodegradation including nature of non-extractable residues (NERs; harmless biogenic [NERsbiogenic] versus hazardous xenobiotic [NERsxenobiotic]) in soils has not been investigated yet. This information is crucial for an assessment of environmental risks related to the speciation of glyphosate-derived NERs which may stem from glyphosate intermediates. Therefore, we incubated 13C- and 15N-labeled glyphosate (2-13C,15N-glyphosate) and its degradation product AMPA (13C,15N-AMPA), sarcosine (13C3,15N-sarcosine) or glycine (13C2,15N-glycine) in an agricultural soil separately for a period of 75 days. 13C2-glycine and 13C3-sarcosine mineralized rapidly compared to 2-13C-glyphosate and 13C-AMPA. The mineralization of 13C-AMPA was lowest among all four compounds due to its persistent nature. Only 0.5% of the initially added 2-13C,15N-glyphosate and still about 30% of the initially added 13C,15N-AMPA was extracted from soil after 75 days. The NERs formed from 13C,15N-AMPA were mostly NERsxenobiotic as compared to other three compounds for which significant amounts of NERsbiogenic were determined. We noticed 2-13C,15N-glyphosate was biodegraded via two biodegradation pathways simultaneously; however, the sarcosine/glycine pathway with the formation of harmless NERsbiogenic presumably dominated.

Microcosm test for pesticide fate assessment in planted water filters: 13C,15N-labeled glyphosate as an example.

Planted filters are often used to remove pesticides from runoff water. However, the detailed fate of pesticides in the planted filters still remains elusive. This hampers an accurate assessment of environmental risks of the pesticides related to their fate and thereby development of proper mitigation strategies. In addition, a test system for the chemical fate analysis including plants and in particular for planted filters is not well established yet. Therefore, we developed a microcosm test to simulate the fate of pesticide in planted filters, and applied 2-13C,15N-glyphosate as a model pesticide. The fate of 2-13C,15N-glyphosate in the planted microcosms over 31 day-incubation period was balanced and compared with that in the unplanted microcosms. The mass balance of 2-13C,15N-glyphosate turnover included 13C mineralization, degradation products, and the 13C and 15N incorporation into the rhizosphere microbial biomass and plants. We observed high removal of glyphosate (> 88%) from the water mainly due to adsorption on gravel in both microcosms. More glyphosate was degraded in the planted microcosms with 4.1% of 13C being mineralized, 1.5% of 13C and 3.8% of 15N being incorporated into microbial biomass. In the unplanted microcosms, 1.1% of 13C from 2-13C,15N-glyphosate was mineralized, and only 0.2% of 13C and 0.1% of 15N were assimilated into microbial biomass. The total recovery of 13C and 15N was 81% and 85% in planted microcosms, and 91% and 93% in unplanted counterparts, respectively. The microcosm test was thus proven to be feasible for mass balance assessments of the fate of non-volatile chemicals in planted filters. The results of such studies could help better manage and design planted filters for pesticide removal.

Microbial community composition and glyphosate degraders of two soils under the influence of temperature, total organic carbon and pH.

Glyphosate can be degraded by soil microorganisms rapidly and is impacted by temperature and soil properties. Enhanced temperature and total organic carbon (TOC) as well as reduced pH increased the rate of 13C315N-glyphosate conversion to CO2 and biogenic non-extractable residues (bioNERs) in a Haplic Chernozem (Muskus et al., 2019) and in a Humic Cambisol (Muskus et al., 2020). To date; however, the combined effect of temperature and TOC or pH on microbial community composition and glyphosate degraders in these two soils has not been investigated. Phospholipid fatty acid [PLFA] biomarker analysis combined with 13C labeling was employed to investigate the effect of two soil properties (pH, TOC) and of three temperatures (10 °C, 20 °C, 30 °C) on soil microorganisms. Before incubation, the properties of a Haplic Chernozem and a Humic Cambisol were adjusted to obtain five treatments: (a) Control (Haplic Chernozem: 2.1% TOC and pH 6.6; Humic Cambisol: 3% TOC and pH 7.0), (b) 3% TOC (Haplic Chernozem) or 4% TOC (Humic Cambisol), (c) 4% TOC (Haplic Chernozem) or 5% TOC (Humic Cambisol), (d) pH 6.0 (Haplic Chernozem) or pH 6.5 (Humic Cambisol), and (e) pH 5.5 for both soils. All treatments were amended with 50 mg kg-1 glyphosate and incubated at 10 °C, 20 °C or 30 °C. We observed an increase in respiration, microbial biomass and glyphosate mineralization with incubation temperature. Although respiration and microbial biomass in the Humic Cambisol was higher, the microorganisms in the Haplic Chernozem were more active in glyphosate degradation. Increased TOC shifted the microbiome and the 13C-glyphosate degraders towards Gram-positive bacteria in both soils. However, the abundance of 13C-PLFAs indicative for the starvation of Gram-negative bacteria increased with increasing TOC or decreasing pH at higher temperatures. Gram-negative bacteria thus may have been involved in earlier stages of glyphosate degradation.

Superabsorbent polymer as a supplement substrate of constructed wetland to retain pesticides from agricultural runoff.

Surface water runoff can export pesticides from agricultural fields into adjacent aquatic ecosystems, where they may pose adverse effects to organisms. Constructed wetlands (CWs) are widely used to treat agricultural runoff contaminated by pesticides, but the removal of hydrophilic pesticides is usually low. In this study, we suggest superabsorbent polymer (SAP), a cross-linked hydrophilic polymer, as a supplement to substrates of CWs and tested the hypothesis that SAP results in an enhanced removal of hydrophilic pesticides. Therefore, batch experiments were conducted to study the retention capacity of water-saturated SAP (w-SAP) for several hydrophilic pesticides. Retention of the pesticides on w-SAP was related to the ionization state and water solubility of the pesticides. The retention of neutral pesticides, imidacloprid, metalaxyl and propiconazole, was about 20% higher than that measured for anionic pesticides, bentazone, glyphosate and MCPA. The retention of the pesticides by w-SAP mainly resulted from their distribution in the gel-water phase of w-SAP, while less water soluble pesticides might have also been adsorbed on the molecular backbone of SAP. Furthermore, we tested the efficacy of w-SAP for treatment of runoff water contaminated by pesticides in lab-scale horizontal subsurface flow CWs. SAP in CWs improved the removal of the pesticides, including the recalcitrant ones. The removal enhancement was owing to the increase of hydraulic retention time and improvement of biodegradation. The removal of the pesticides in SAP containing CWs was > 93% for MCPA, glyphosate, and propiconazole, 62 - 99% for imidacloprid, 50 - 84% for metalaxyl, and 38 - 73% for bentazone. In the control gravel CWs, the removal was > 98% for glyphosate, generally > 83% for MCPA and propiconazole, 46 - 98% for imidacloprid, 32 - 97% for metalaxyl, and 9 - 96% for bentazone.

Retention and distribution of pesticides in planted filter microcosms designed for treatment of agricultural surface runoff.

Pesticides in agricultural surface water runoff cause a major threat to freshwater systems. Installation of filter systems or constructed wetlands in areas of preferential run-off is a possible measure for pesticides abatement. To develop such systems, combinations of filter materials suitable for retention of both hydrophilic and hydrophobic organic pesticides were tested for pesticide removal in planted microcosms. The retention of six pesticides frequently detected in surface waters (bentazone, MCPA, metalaxyl, propiconazole, pencycuron, and imidacloprid) was evaluated in unplanted and planted pot experiments with novel bed material mixtures consisting of pumice, vermiculite, water super-absorbent polymer (SAP) for retention of ionic and water soluble pesticides, and synthetic hydrophobic wool for adsorption of hydrophobic pesticides. The novel materials were compared to soil with high organic matter content. The highest retention of the pesticides was observed in the soil, with a considerable translocation of pesticides into the plants, and low leaching potential, in particular for the hydrophobic compounds. However, due to the high retention of pesticides in soil, environmental risks related to their long term mobilization cannot be excluded. Mixtures of pumice and vermiculite with SAP resulted in high retention of i) water and ii) both hydrophilic and hydrophobic pesticides but with much lower leaching potential compared to the mineral systems without SAP. Mixtures of such materials may provide near natural treatment options in riparian strips and also for treatment of rainwater runoff without the need for water containment systems.

ENSAT registry-based randomized clinical trials for adrenocortical carcinoma.

Adrenocortical carcinoma (ACC) is an orphan disease lacking effective systemic treatment options. The low incidence of the disease and high cost of clinical trials are major obstacles in the search for improved treatment strategies. As a novel approach, registry-based clinical trials have been introduced in clinical research, so allowing for significant cost reduction, but without compromising scientific benefit. Herein, we describe how the European Network for the Study of Adrenal Tumours (ENSAT) could transform its current registry into one fit for a clinical trial infrastructure. The rationale to perform randomized registry-based trials in ACC is outlined including an analysis of relevant limitations and challenges. We summarize a survey on this concept among ENSAT members who expressed a strong interest in the concept and rated its scientific potential as high. Legal aspects, including ethical approval of registry-based randomization were identified as potential obstacles. Finally, we describe three potential randomized registry-based clinical trials in an adjuvant setting and for advanced disease with a high potential to be executed within the framework of an advanced ENSAT registry. Thus we, therefore, provide the basis for future registry-based trials for ACC patients. This could ultimately provide proof-of-principle of how to perform more effective randomized trials for an orphan disease.

Fate of pendimethalin in soil and characterization of non-extractable residues (NER).

One important route of degradation of herbicide pendimethalin in soil leads to formation of non-extractable residues (NER). To investigate NER nature (irreversibly, chemically bound, including possible biogenic NER, or strongly sorbed and entrapped) residues of 14C-labelled pendimethalin in soil were investigated after conventional extraction with organic solvents by silylation. After 400 days of incubation, 32.0% of applied radioactivity (AR) was transformed into NER, 39.9% AR remained extractable. Mineralization reached 26.2% AR. Additionally, 14C-pendimethalin was incubated in soil amended with compost for 217 days to investigate the influence of organic amendments on NER formation. NER amounted to 37.8% AR, with 57.9% AR remaining extractable. Mineralization was negligible (1.4% AR). For all sampling times only low amounts of radioactivity were entrapped (<5% AR) in soil without compost amendment. Pendimethalin was present only in trace amounts (ca. 0.4% AR), other released residues consisted of undefined fractions (sum ≈2% AR). In soil amended with compost, silylation overall resulted in release of higher amounts of radioactivity (19% AR). Addition of compost led to an increase in potential entrapment and sorption sites for pendimethalin, forming higher amounts of strongly sorbed, entrapped residues. Furthermore, potential release of non-extractable pendimethalin residues was investigated by incubation of solvent-extracted soil (without compost amendment) mixed with fresh soil for additional 3 months. NER were partly mineralized (7% AR) and 20% became extractable with organic solvents. However, no pendimethalin or any known metabolites were found. It can be concluded that no parent pendimethalin was found and NER of pendimethalin in soil are mainly formed by covalent binding to organic matrix with only low potential of remobilization under natural conditions.

High prevalence of steroid-induced glucose intolerance with normal fasting glycaemia during low-dose glucocorticoid therapy: an oral glucose tolerance test screening study.

OBJECTIVES: To evaluate the prevalence and risk factors of new-onset glucose metabolism impairment using an oral glucose tolerance test (OGTT) in patients with normal fasting glycaemia on long-term glucocorticoid (GC) treatment. METHODS: An OGTT was performed in 150 patients without a previous history of pre-diabetes or diabetes who were diagnosed with inflammatory rheumatic diseases and treated with GCs >3 months. All participants underwent clinical and biochemical evaluation for risk factors of diabetes: age, sex, current and cumulative dose of steroids, treatment duration, waist circumference, BMI, Homeostatic Model Assessment for Insulin Resistance, fasting insulin concentration, family history of diabetes, CRP, 28-joint DAS with CRP, type of connective tissue disease and trunk fat percentage measured by DXA. Logistic regression analysis was conducted to evaluate the association between the presence of impaired glucose tolerance (IGT) in the OGTT and analysed risk factors. RESULTS: A total of 102 patients (68%) had fully normal glucose tolerance. Diabetes, isolated impaired fasting glucose, isolated IGT and combined impaired fasting glucose + IGT was diagnosed in 3.3, 4.67, 19.33 and 4.67% of patients, respectively; 20% of participants had IGT or diabetes despite normal fasting glucose concentration. The median cumulative dose and current dose (5 mg) of GCs and treatment duration were similar compared with the normal glucose tolerance group. In a multivariate logistic regression model, only older age (particularly ≥50 years of age) and trunk fat percentage remained significant factors predicting IGT or diabetes in the OGTT. CONCLUSION: New-onset GC-induced glucose intolerance, even in patients on long-term low-dose treatment, is prevalent despite normal fasting glucose concentration and patients should be screened with an OGTT despite the absence of classic risk factors of diabetes.

Pitfalls in the diagnostics of aldosterone-producing adrenocortical carcinoma.

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Plant litter enhances degradation of the herbicide MCPA and increases formation of biogenic non-extractable residues in soil.

Amendment of soils with plant residues is common practice for improving soil quality. In addition to stimulated microbial activity, the supply of fresh soluble organic (C) from litter may accelerate the microbial degradation of chemicals in soils. Therefore, the aim of this study was to test whether the maize litter enhances degradation of 4-chloro-2-methylphenoxyacetic acid (MCPA) and increases formation of non-toxic biogenic non-extractable residues (bioNERs). Soil was amended with 13C6-MCPA and incubated with or without litter addition on the top. Three soil layers were sampled with increasing distance from the top: 0-2 mm, 2-5 mm and 5-20 mm; and the mass balance of 13C6-MCPA transformation determined. Maize litter promoted microbial activity, mineralization of 13C6-MCPA and bioNER formation in the upper two layers (0-2 and 2-5 mm). The mineralization of 13C6-MCPA in soil with litter increased to 27% compared to only 6% in the control. Accordingly, maize addition reduced the amount of extractable residual MCPA in soil from 77% (control) to 35% of initially applied 13C6-MCPA. While non-extractable residues (NERs) were <6% in control soil, litter addition raised NERs to 21%. Thereby, bioNERs comprised 14% of 13C6-MCPA equivalents. We found characteristic differences of bioNER formation with distance to litter. While total NERs in soil at a distance of 2-5 mm were mostly identified as 13C-bioNERs (97%), only 45-46% of total NERs were assigned to bioNERs in the 0-2 and 5-20 mm layers. Phospholipid fatty acid analysis indicated that fungi and Gram-negative bacteria were mainly involved in MCPA degradation. Maize-C particularly stimulated fungal activity in the adjacent soil, which presumably facilitated non-biogenic NER formation. The plant litter accelerated formation of both non-toxic bioNERs and non-biogenic NERs. More studies on the structural composition of non-biogenic NERs with toxicity potential are needed for future recommendations on litter addition in agriculture.

Corticotropinoma as the underlying cause of intermittent Cushing's syndrome in a patient previously diagnosed with primary pigmented nodular adrenocortical disease.

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Degradation of glyphosate in a Colombian soil is influenced by temperature, total organic carbon content and pH.

Glyphosate is one of the most used herbicides in the world. The fate of glyphosate in tropical soils may be different from that in soils from temperate regions. In particular, the amounts and types of non-extractable residues (NER) may differ considerably, resulting in different relative contributions of xenoNER (sorbed and sequestered parent compound) and bioNER (biomass residues of degraders). In addition, environmental conditions and agricultural practices leading to total organic carbon (TOC) or pH variation can alter the degradation of glyphosate. The aim of this study is thus to investigate how the glyphosate degradation and turnover are influenced by varying temperature, pH and TOC of sandy loam soil from Colombia. The pH or TOC of a Colombian soil was modified to yield five treatments: control (pH 7.0, TOC 3%), 4% TOC, 5% TOC, pH 6.5, and pH 5.5. Each treatment received 50 mg kg-1 of 13C315N-glyphosate and was incubated at 10 °C, 20 °C and 30 °C for 40 days. Rising temperature increased the mineralization of 13C315N-glyphosate from 13 to 20% (10 °C) to 32-39% (20 °C) and 41-51% (30 °C) and decreased the amounts of extractable 13C315N-glyphosate after 40 days of incubation from 13 to 26% (10 °C) to 4.6-12% (20 °C) and 1.2-3.2% (30 °C). Extractable 13C315N-glyphosate increased with higher TOC and higher pH. Total 13C-NER were similar in all treatments and at all temperatures (47%-60%), indicating that none of the factors studied affected the amount of total 13C-NER. However, 13C-bioNER dominated within the 13C-NER pool in the control and the 4% TOC treatment (76-88% of total 13C-NER at 20 °C and 30 °C), whereas in soil with 5% TOC and pH 6.5 or 5.5 13C-bioNER were lower (47-61% at 20 °C and 30 °C). In contrast, the 15N-bioNER pool was small (between 14 and 39% of the 15N-NER). Thus, more than 60% of 15N-NER is potentially hazardous xenobiotic NER which need careful attention in the future.

Perceptual audio processing stethoscope.

Stethoscopes are used to transmit body sounds related to various physiological processes to ears of a physician, providing basic or supportive information for eventual diagnosis. Unfavorably, the dominant frequency components of most of the auscultation signals are localized close to the lower frequency limits of the human auditory system, restricting the achievable selectivity and specificity. The present study introduces an approach that aims at overcoming the existing limitations. A signal processing scheme utilizing knock rejection, dynamic compressor, and pseudo-stereo synthesizer blocks is described, along with hardware implementation and results of the initial subjective evaluation.

Microbial Turnover of Glyphosate to Biomass: Utilization as Nutrient Source and Formation of AMPA and Biogenic NER in an OECD 308 Test.

Environmental fate assessment of chemicals involves standardized simulation tests with isotope-labeled molecules to balance transformation, mineralization, and formation of nonextractable residues (NER). Methods to predict microbial turnover and biogenic NER have been developed, having limited use when metabolites accumulate, the chemicals are not the only C source, or provide for other macroelements. To improve predictive capability, we extended a recently developed method for microbial growth yield estimation to account for incomplete degradation and multiple-element assimilation and combined it with a dynamic model for fate description in soils and sediments. We evaluated the results against the unique experimental data of 13C3-15N co-labeled glyphosate turnover with AMPA formation in water-sediment systems (OECD 308). Balancing 13C- and 15N- fluxes to biomass showed a pronounced shift of glyphosate transformation from full mineralization to AMPA formation. This may be explained by various hypotheses, for example, the limited substrate turnover inherent to the batch conditions of the test system causing microbial starvation or inhibition by P release. Modeling results indicate initial N overload due to the lower C/N ratio in glyphosate compared to average cell composition leading to subsequent C demand and accumulation of AMPA.

Low-dose etomidate for the management of severe hypercortisolaemia in different clinical scenarios: a case series and review of the literature.

BACKGROUND: Severe Cushing's syndrome (SCS) is associated with acute cardiovascular, metabolic and infectious complications. It is considered an emergency, requiring an immediate diagnosis, together with a broad spectrum of supportive and hypocortisolaemic treatments. Surgical intervention, aimed at removing the source of cortisol or adrenocorticotropic hormone (ACTH), is the optimal treatment in most cases of Cushing's syndrome. However, in hypercortisolaemic states, surgical intervention has high rates of perioperative mortality and morbidity. Oral adrenal steroidogenesis inhibitors, even if more effective in combination, are not always efficient enough or well tolerated. Despite their common use, a more potent, parental, immediate, and thus life-saving, therapy is necessary. METHODS: The authors present three different clinical scenarios of etomidate treatment in patients hospitalized in the third reference endocrinological centre in Poland between 2016 and 2017. RESULTS: Patients with Cushing's disease, ectopic Cushing's syndrome and adrenocortical carcinoma presented with severe hypercortisolaemia and exacerbated cortisol-dependent comorbidities. In these three cases, etomidate acted as an accurate, well tolerated and effective cortisol-lowering drug for several days or even months. Patients were monitored in a general ward setting, and no side effects of the therapy were observed. CONCLUSIONS: In doses far lower than those used for anaesthesia, etomidate works as a useful cortisol-lowering therapy in patients intolerant to or unable to take oral medications. Additionally, if urgent, the most potent and effective medical intervention is necessary, and clinicians should be aware of such a therapeutic option.

Effect of temperature, pH and total organic carbon variations on microbial turnover of 13C315N-glyphosate in agricultural soil.

Glyphosate is the best-selling and the most-used broad-spectrum herbicide worldwide. Microbial conversion of glyphosate to CO2 and biogenic non-extractable residues (bioNER) leads to its complete degradation. The degradation of glyphosate may vary in different soils and it depends on environmental conditions and soil properties. To date, the influence of temperature, soil pH and total organic carbon (TOC) on microbial conversion of glyphosate to bioNER has not been investigated yet. The pH or TOC of an agricultural original soil (pH 6.6, TOC 2.1%) was modified using sulfuric acid or farmyard manure (FYM), respectively. Each treatment: original (I), 3% TOC (II), 4% TOC (III), pH 6.0 (IV) and pH 5.5 (V) was amended with 13C315N-glyphosate and incubated at 10 °C, 20 °C and 30 °C for 39 days. The temperature was the main factor controlling the mineralization and the extractable 13C315N-glyphosate, whereas higher TOC content and lower pH resulted in enhanced formation of 13C-bioNER. After 39 days the cumulative mineralization of 13C-glyphosate was in the range of 12-22% (10 °C), 37-47% (20 °C) and 43-54% (30 °C). Extractable residues of 13C-glyphosate were in the range of 10-21% (10 °C) and 4-10% (20 °C and 30 °C); whereas those of 15N-glyphosate were as follows 20-32% (10 °C) and 12-25% (20 °C and 30 °C). The 13C-NER comprised about 53-69% of 13C-mass balance in soils incubated at 10 °C, but 40-50% in soils incubated at 20 °C and 30 °C. The 15N-NER were higher than the 13C-NER and varied between 62% and 74% at 10 °C, between 53% and 81% at 20 °C and 30 °C. A major formation of 13C-bioNER (72-88% of 13C-NER) at 20 °C and 30 °C was noted in soil amended with FYM. An increased formation of 15N-bioNER (14-17% of 15N-NER) was also observed in FYM-amended soil. The xenobiotic 15N-NER had a major share within the 15N-NER and thus need to be considered when assessing the environmental risk of glyphosate-NER.